New tests for bile and detection of bile in serum and urine.

HE PURPOSES OF THE PRESENT PAPER are to describe some new sensitive tests suitable for the detection of bile in biologic fluids which overcome the defects demonstrated in procedures described in most standard texts, and also to confirm the experiments of C. E. May et at. (1). The sensitivity of the proposed oxidation tests to detect bilirubin, viz. ferricyanide, molybdate, N-bromosuccinimide (2, 3), N-chlorosuccinimide (4), and dichromate tests, is compared with the previously known Gmelin, Fouchet, Obermeyer, Nakayama, iodine and nitrite tests. Similarly a comparison of the proposed diazo tests, i.e., diazochlorides obtained from various members of sulfonamides, as well as from the aromatic amines previously tested by May et at. (1), with the previously known diazotized sulfanilic acid test is reported.